DTT CAS 3483-12-3 DL-Dithiothreitol Biochemical Reagents Powder manufacturers and suppliers in China

DTT CAS 3483-12-3 DL-Dithiothreitol Biochemical Reagents Powder

Description

Name:Form:Color:Storage Temp:Reaction Suitability:Samples:Package:Assay:High Light:

DL-Dithiothreitol	Powder
White	2-8°C
Reagent Type: Reductant	Available
Bottle/Poly Bottle/Drum	98%
3483-12-3 DL-Dithiothreitol , DL-Dithiothreitol Biochemical Reagents Powder

DTT CAS 3483-12-3 DL-Dithiothreitol Biochemical Reagents Powder

DL-Dithiothreitol Basic information

DL-Dithiothreitol Basic information


DL-Dithiothreitol Chemical Properties

DL-Dithiothreitol Chemical Properties

Melting point	41-44 °C(lit.)
alpha	-0.2～+0.2°(20℃/D)(c=5,H2O)
Boiling point	125 °C
density	1.04 g/mL at 20 °C
vapor density	5.3 (vs air)
refractive index	1.5200 (estimate)
Fp	>230 °F
storage temp.	-20°C (or 4°C short term).
solubility	H2O: 50 mg/mL, clear, colorless
pka	pK1:8.9 (25°C)
form	Powder
color	White
Odor	Unpleasant Odor
PH	4.0-6.0 (20-25℃, 0.1m in H2O)
PH Range	4 - 6 at 15,4 g/l at 25 °C
Water Solubility	freely soluble
λmax	λ: 260 nm Amax: 0.400 λ: 280 nm Amax: 0.100
Sensitive	Air Sensitive
Merck	14,3376
BRN	1719757
Stability:	Stability Stable, but heat sensitive. Incompatible with strong oxidizing agents. Keep frozen at -20 to -10 C.
InChIKey	VHJLVAABSRFDPM-UHFFFAOYSA-N
CAS DataBase Reference	3483-12-3(CAS DataBase Reference)
NIST Chemistry Reference	2,3-Butanediol, 1,4-dimercapto-, (r,r)-(3483-12-3)
EPA Substance Registry System	1,4-Dithiothreitol (3483-12-3)

DL-Dithiothreitol Usage And Synthesis

DL-Dithiothreitol Usage And Synthesis

Chemical Properties	White solid
Uses	Reagent for the quantitative reduction of disulfide group. Prevents the oxidation of cholecystokinin during its extraction from brain and duodenal tissues. Cleavage of disulfide bonds in proteins.
Uses	DTT is a cell permeable protective agent for Thiol groups.
Purification Methods	Crystallise DTT from ether and sublime it at 37o/0.005mm. It should be stored at 0o. [Beilstein 1 III 2360.]
General Description	An excellent reagent for maintaining SH groups in reduced state; quantitatively reduces disulfides. DTT is effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N′-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol. Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).